rabbit anti human gp96 (Boster Bio)
Structured Review

Rabbit Anti Human Gp96, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+human+gp96/pmc08767371-39-5-8?v=Boster+Bio
Average 91 stars, based on 3 article reviews
Images
1) Product Images from "GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma"
Article Title: GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma
Journal: Journal of Healthcare Engineering
doi: 10.1155/2022/2518847
Figure Legend Snippet: Lentiviral vectors: LV5: GP96 lentiviral vector structure; LV8: SMP30 lentiviral vector structure. (a) GP96-transfected DCs. (b) SMP30-transfected DCs. (a) and (b) show the same location.
Techniques Used: Plasmid Preparation, Transfection
Figure Legend Snippet: WB after transfection. (a) Detection of GP96 in 6 groups. (b) Detection of SMP30 in 6 groups. (c) GAPDH as the internal control. The GP96 group expressed more GP96, and the SMP30 group expressed more SMP30, indicating that transfection was successful.
Techniques Used: Transfection, Control
Figure Legend Snippet: Liver cancer model. (a) The liver cancer model. (b) The GP96 + SMP30 group: tumors had disappeared in some mice on day 4. (c) Tumors harvested from mice. (d) Negative TUNEL staining for apoptosis in the DC group. (e) Positive TUNEL staining for apoptosis in the GP96 + SMP30 group.
Techniques Used: TUNEL Assay, Staining
Figure Legend Snippet: Tumor growth curve and ELISA results. Curve: the tumor volume in the GP96 + SMP30 group was smaller than that in the protein group on day 4, day 14, and day 16, P < 0.05. IL-2: the GP96 + SMP30 group showed secretion of more IL-2 than the other groups, P < 0.01. IFN- γ : the GP96 + SMP30 group showed secretion of more IFN- γ than the other groups, P < 0.01.
Techniques Used: Enzyme-linked Immunosorbent Assay
Figure Legend Snippet: Immunohistochemical staining of tumors. (a) Tumor expressing IL-2 in the GP96 + SMP30 group; the brown cytoplasm indicates positive staining. (b) Tumor expressing IL-2 in the GP96 group; the colorless cytoplasm indicates negative staining. (c) Tumor expressing IFN- γ in the GP96 + SMP30 group; the brown cytoplasm indicates positive staining. (d) Tumor expressing IFN- γ in the SMP30 group; the colorless cytoplasm indicates negative staining.
Techniques Used: Immunohistochemical staining, Staining, Expressing, Negative Staining